Peptide Calculator

Reconstitution and dosing math in one place: how much bacteriostatic water to add, and where to draw on the syringe. Free, no signup.

Reviewed for accuracy by Simon Chang, BScPharm · Last reviewed 18 October 2026

For research reference only. The math assumes your vial contains what its label claims — check its published batch result first.

RESULT
40 units
= 0.40 ml on a U-100 insulin syringe
Concentration5.0 mg/ml
Doses in this vial5

The calculator above converts vial size, water volume, and dose into exact syringe units for any peptide. Below: how the math works, which syringe to use, and the one assumption no calculator can check for you.

How the math works

  1. Concentration: vial mg ÷ water ml. A 10mg vial with 2ml of bacteriostatic water is 5 mg per ml.
  2. Volume per dose: dose mg ÷ concentration. A 2mg dose at 5 mg/ml is 0.4 ml of solution.
  3. Syringe units: on a U-100 insulin syringe, 1 ml = 100 units, so 0.4 ml means drawing to the 40-unit mark.

Quick reference: 10mg vial

Water added Concentration 1mg dose 2mg dose 5mg dose
1 ml 10 mg/ml 10 units 20 units 50 units
2 ml 5 mg/ml 20 units 40 units 100 units
3 ml 3.3 mg/ml 30 units 60 units exceeds syringe

How do you keep reconstitution sterile and dispose of needles safely?

Use a new sterile needle and syringe for every draw, wipe each vial stopper with an alcohol swab before puncturing it, and put used sharps straight into a sharps disposal container. Infection risk comes from handling, not from the arithmetic, so these steps matter as much as the numbers above.

  • One needle, one syringe, one use. Never reuse a needle or syringe, and never share one (CDC injection safety guidance).
  • Clean the stopper every time. Wipe the rubber stopper of the water vial and the peptide vial with an alcohol swab before each puncture.
  • Do not leave a needle in the vial. CDC advises against leaving a needle inserted in a vial stopper for repeated draws.
  • Date the water vial. Once a multiple-dose vial such as bacteriostatic water has been punctured, CDC guidance is to date it and discard it within 28 days unless the manufacturer states another date.
  • Dispose of sharps immediately. Place used needles and syringes in a sharps disposal container right after use, never loose in household trash or recycling. When the container is about three-quarters full, follow your local rules for getting rid of it (FDA sharps disposal guidance).

Which syringe should you use?

The draw volume decides the syringe, and the calculator shows your draw volume instantly. Match it to the smallest syringe that fits:

Syringe Best for draws of Notes
0.3 ml U-100 insulin under 0.25 ml Finest graduations; the accuracy pick for small doses of potent compounds
0.5 ml U-100 insulin 0.25 to 0.45 ml The workhorse for most reconstitution setups
1 ml U-100 insulin 0.45 to 1 ml Needed when low concentrations push draws close to a full ml
3 ml syringe reconstitution only Graduated in ml rather than units; right for adding bacteriostatic water to the vial, wrong for measuring doses under 0.5 ml

The common mistake behind “peptide calculator for a 3ml syringe” searches: a 3ml barrel is a transfer tool. Use it to add water, then dose with an insulin syringe sized to your draw. If your calculated draw exceeds 1 ml, add less water next time to raise the concentration instead of reaching for a bigger barrel.

Milligrams, micrograms, and units: the three vocabularies

Dosing discussions mix three unit systems, and most dosing errors are unit errors:

  • mg ↔ mcg: 1 mg = 1,000 mcg. A 250 mcg dose is 0.25 mg; the calculator accepts either once you convert.
  • Syringe units are volume, not dose: “40 units” means 0.4 ml on a U-100 syringe. How much compound that contains depends entirely on your concentration. That is why “how many units should I draw?” has no answer without knowing vial size and water added.
  • U-100 vs U-40: the number is units per ml. The same 0.4 ml draw reads 40 units on a U-100 but 16 units on a U-40. Mixing them up is a 2.5x dosing error, which is why the calculator asks.

The math is only as good as the vial

This calculator assumes your 10mg vial holds 10mg. Published third-party testing shows label claims regularly miss in this market: failed results turn up regularly in Janoshik’s public results database. Check your vendor’s published batch results on their PeptideCensus profile before relying on the label.

Common questions

How much water should I add to a 10mg vial?

There is no single right answer. More water means lower concentration and larger, easier-to-measure draws. 2ml on a 10mg vial (5 mg/ml) is a common middle ground in research protocols; small doses of potent compounds measure more accurately at lower concentrations.

How many units is 5mg?

Impossible to answer without concentration; that’s the point of the calculator. At 5 mg/ml, a 5mg dose is 1 ml = 100 units on a U-100; at 10 mg/ml it’s 0.5 ml = 50 units.

Does the calculator work for any peptide?

Yes: the reconstitution math is identical for retatrutide, tirzepatide, BPC-157, TB-500, or anything else in a lyophilized vial. Compound-specific context lives on each peptide hub.

Can I use a regular syringe instead of an insulin syringe?

The math still works in ml, but insulin syringes exist because sub-1ml volumes need fine graduations; a 3ml syringe measuring a 0.15ml draw is guesswork.

Can I use a 3ml syringe with this calculator?

For reconstitution, yes: measure your bacteriostatic water with it. For dosing, convert to an insulin syringe; the calculator’s unit output is for insulin-syringe graduations (U-100 or U-40), and a 3ml barrel cannot resolve a 0.2 ml draw accurately.

How long does a reconstituted vial last?

With bacteriostatic water and refrigeration, research protocols commonly work within a several-week window, but stability varies by compound. Check the peptide’s hub page, and when in doubt, reconstitute smaller vials.

Does the math change if my vial is underdosed?

Yes, proportionally. A “10mg” vial that tested at 8mg makes every calculated dose 20% lighter than intended. That is why batch results matter more than arithmetic.